Determination of Forsythin in Yanbao Mixture by HPLC

Abstract: Yanbao Mixture is a prescription under clinical agreement. It has the effects of clearing away heat and detoxifying, and relieving pharyngeal phlegm. It is mainly used for acute pharyngitis, acute tonsillitis and upper respiratory tract infections. In order to better control the quality of this variety, the author formulated the preparation process and studied its quality control methods.

Objective To prepare Yanbao mixture and control its quality. Methods TLC was used to identify forsythia; forsythin was controlled by high performance liquid chromatography. The column was EclipseXDB-C18 (250mmX4.6mm, 5μm), mobile phase: chloroform-methanol (6: 1) , Detection wavelength: 277nm. Results Forsythiaside showed a good linear relationship within the concentration range of 0.1-0.5g / L, r = 0.9999, the average recovery rate was 99.01%, and RSD = 0.86% (n = 5). Conclusion The preparation process is simple and feasible, and the content determination is fast and accurate, which can be used for the quality control of the preparation.

1 Instruments and reagents

LC—2010AHT high-performance liquid chromatograph (Japan Shimadzu Corporation); LCsolution chromatographic workstation. Forsythiaside reference substance (China National Institute for the Control of Pharmaceutical and Biological Products); Yanbao Mixture (specification 100ml); reagents are of analytical grade.

2 Prescription and preparation

2.1 Prescription

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